论文标题
ATP驱动的细菌中液相冷凝水的分离
ATP-driven separation of liquid phase condensates in bacteria
论文作者
论文摘要
在没有膜的情况下，液态液相分离（LLP）状态是隔室分量的关键，但是尚不清楚LLPS冷凝物是否在亚细胞空间以及通过哪种机制中进行了积极和专门组织。在这里，我们通过重点关注由层状序列（PARS），DNA结合蛋白（PARB）和运动（Para）组成的Parabs DNA分离系统来解决这个问题。我们表明，PARS-PARB合伙人形成纳米大小的圆形冷凝物。 PARB分子在核苷体积内迅速扩散，但是当被困在PARB冷凝水中时显示出限制运动。单个PARB分子能够在不同的冷凝物之间迅速扩散，而成核受到PARS的强烈青睐。值得注意的是，需要Para电动机以防止PARB冷凝物的融合。这些结果描述了一种新型的活跃机制，该机制将亚细胞空间中的非典型LLP分割，分离和局部。
Liquid-liquid phase separated (LLPS) states are key to compartmentalise components in the absence of membranes, however it is unclear whether LLPS condensates are actively and specifically organized in the sub-cellular space and by which mechanisms. Here, we address this question by focusing on the ParABS DNA segregation system, composed of a centromeric-like sequence (parS), a DNA-binding protein (ParB) and a motor (ParA). We show that parS-ParB associate to form nanometer-sized, round condensates. ParB molecules diffuse rapidly within the nucleoid volume, but display confined motions when trapped inside ParB condensates. Single ParB molecules are able to rapidly diffuse between different condensates, and nucleation is strongly favoured by parS. Notably, the ParA motor is required to prevent the fusion of ParB condensates. These results describe a novel active mechanism that splits, segregates and localises non-canonical LLPS condensates in the sub-cellular space.
