论文标题
在红色（620 nm）光照射下，光培养基的频谱动力学建模和长期行为评估在光生反应器中生长
Spectral Kinetic Modeling and Long-term Behavior Assessment of Arthrospira platensis Growth in Photobioreactor under Red (620 nm) Light Illumination
论文作者
论文摘要
研究了使用高能量效率（准单色）RED LED培养人为减轻光生反应器中蓝细菌量角杆菌的能力。为了达到与多色闪电控制条件（红色 +蓝色，p/2e- = 1.275）相同的最大生产率，首先在批处理和持续培养物上建立了与620 nm左右的最佳波长范围一起工作的需求。然后，在连续的光生反应器中验证了长期的生理和动力学行为，仅通过红色（620 nm）LED照明，表明可以在30个停留时间内保持最大的生产率，仅在30个停留时间内，只有较小的细胞色素含量变化，而对应于众所周知的适应机制，但对生长和Stoichiiement却没有任何影响。对于聚和单色入射光输入，提出并首次提出了预测光谱知识模型，并允许在任何光生反应器培养的植物A. platensis或其他蓝细菌中观察到的动力学和化学计量计算，如果参数已更新，则可以计算。结果表明，必须使用光子通量（具有指定波长）代替光能通量作为生长的相关控制变量。这项研究中获得的实验和理论结果表明，使用红色LED的光生反应器的亮光设备可以节省消耗的能量，其光谱范围是根据光合作用的动作谱定义的。对于必须合理化能量的应用，这似乎是至关重要的信息，因为在封闭环境中，诸如永久空间基础或潜艇等封闭环境中的生命支持系统就是这种情况。
The ability to cultivate the cyanobacterium Arhtrospira platensis in artificially lightened photobioreactors using high energetic efficiency (quasi-monochromatic) red LED was investigated. In order to reach the same maximal productivities as with the polychromatic lightening control conditions (red + blue, P/2e- = 1.275), the need to work with an optimal range of wavelength around 620 nm was first established on batch and continuous cultures. The long-term physiological and kinetic behavior was then verified in a continuous photobioreactor illuminated only with red (620 nm) LED, showing that the maximum productivities can be maintained over 30 residence times with only minor changes in the pigment content of the cells corresponding to a well-known adaptation mechanism of the photosystems, but without any effect on growth and stoichiometry. For both poly and monochromatic incident light inputs, a predictive spectral knowledge model was proposed and validated for the first time, allowing the calculation of the kinetics and stoichiometry observed in any photobioreactor cultivating A. platensis, or other cyanobacteria if the parameters were updated. It is shown that the photon flux (with a specified wavelength) must be used instead of light energy flux as a relevant control variable for the growth. The experimental and theoretical results obtained in this study demonstrate that it is possible to save the energy consumed by the lightening device of photobioreactors using red LED, the spectral range of which is defined according to the action spectrum of photosynthesis. This appears to be crucial information for applications in which the energy must be rationalized, as it is the case for life support systems in closed environments like a permanent spatial base or a submarine.
