论文标题
使用三维动态光学相干断层扫描
Label-free drug response evaluation of human derived tumor spheroids using three-dimensional dynamic optical coherence tomography
论文作者
论文摘要
我们通过动态光学相干断层扫描（OCT）展示了人乳腺（MCF-7）和结肠（HT-29）癌球体的无标记药物反应评估。 MCF-7和HT-29球体分别用紫杉醇（PTX或紫杉醇）和Irinotecan（SN-38）的活性代谢物处理。使用0（对照），0.1、1和10 UM浓度使用药物，治疗时间为1、3和6天。使用重复的栅格扫描方案扫描样品，并应用两种动态OCT算法，对数强度方差（LIV）和OCT相关衰减速度（OCDSL）分析，以可视化组织和细胞动力学。清晰可视化两种球体类型的不同药物反应模式，并通过LIV和OCDSL成像进行定量分析。对于两种球体类型，都观察到结构性腐败以及LIV和OCDS的减少。这些结果可能表明药物作用的不同机制。结果表明，动态OCT可用于突出药物反应模式并进行抗癌药物测试。
We demonstrate label-free drug response evaluations of human breast (MCF-7) and colon (HT-29) cancer spheroids via dynamic optical coherence tomography (OCT). The MCF-7 and HT-29 spheroids were treated with paclitaxel (PTX, or Taxol) and the active metabolite of irinotecan (SN-38), respectively. The drugs were applied using 0 (control), 0.1, 1, and 10 uM concentrations with treatment times of 1, 3, and 6 days. The samples were scanned using a repeated raster scan protocol and two dynamic OCT algorithms, logarithmic intensity variance (LIV) and late OCT correlation decay speed (OCDSl) analyses, were applied to visualize the tissue and cellular dynamics. Different drug response patterns of the two spheroid types were visualized clearly and analyzed quantitatively by LIV and OCDSl imaging. For both spheroid types, structural corruptions and reduction of LIV and OCDSl were observed. These results may indicate different mechanisms of the drug action. The results suggest that dynamic OCT can be used to highlight drug response patterns and perform anti-cancer drug testing.
